Proinflammatory and cytotoxic response to nanoparticles in precision-cut lung slices

• Stephanie Hirn,
• Nadine Haberl,
• Kateryna Loza,
• Matthias Epple,
• Wolfgang G. Kreyling,
• Barbara Rothen-Rutishauser,
• Markus Rehberg and
• Fritz Krombach

Beilstein J. Nanotechnol. 2014, 5, 2440–2449, doi:10.3762/bjnano.5.253

• Papeterie CP 209, 1723 Marly, Switzerland 10.3762/bjnano.5.253 Abstract Precision-cut lung slices (PCLS) are an established ex vivo alternative to in vivo experiments in pharmacotoxicology. The aim of this study was to evaluate the potential of PCLS as a tool in nanotoxicology studies. Silver (Ag-NPs) and

• types and concentrations need to be tested in further studies. Keywords: cytokines; cytotoxicity; ex vivo; lung slices; nanoparticles; Introduction Nanoparticles (NPs) are defined as materials with one dimension between 1–100 nm that occur naturally or anthropogenically. The class of synthetic NPs can

• slices can be an alternative to in vitro and in vivo studies [4]. Precision-cut lung slices (PCLS) are already used as an alternative ex vivo model in lung pharmacotoxicology [5]. As the lungs are also an important primary target organ for aerosolized ENPs, PCLS could have the potential to serve as an

PVP-coated, negatively charged silver nanoparticles: A multi-center study of their physicochemical characteristics, cell culture and in vivo experiments

• Sebastian Ahlberg,
• Alexandra Antonopulos,
• Jörg Diendorf,
• Ralf Dringen,
• Matthias Epple,
• Rebekka Flöck,
• Wolfgang Goedecke,
• Christina Graf,
• Nadine Haberl,
• Jens Helmlinger,
• Fabian Herzog,
• Frederike Heuer,
• Stephanie Hirn,
• Christian Johannes,
• Stefanie Kittler,
• Manfred Köller,
• Katrin Korn,
• Wolfgang G. Kreyling,
• Fritz Krombach,
• Jürgen Lademann,
• Kateryna Loza,
• Eva M. Luther,
• Marcelina Malissek,
• Martina C. Meinke,
• Daniel Nordmeyer,
• Anne Pailliart,
• Jörg Raabe,
• Fiorenza Rancan,
• Barbara Rothen-Rutishauser,
• Eckart Rühl,
• Carsten Schleh,
• Andreas Seibel,
• Christina Sengstock,
• Lennart Treuel,
• Annika Vogt,
• Katrin Weber and
• Reinhard Zellner

Beilstein J. Nanotechnol. 2014, 5, 1944–1965, doi:10.3762/bjnano.5.205

• silver nanoparticles in vivo induced a moderate pulmonary toxicity, however, only at rather high concentrations. The same was found in precision-cut lung slices of rats in which silver nanoparticles remained mainly at the tissue surface. In a human 3D triple-cell culture model consisting of three cell

• intratracheal instillation can cause moderate pulmonary toxicity in vivo, but only at rather high concentrations [124]. Ex vivo approaches, such as isolated-perfused lungs or precision-cut lung slices (PCLS), have been developed as an alternative to in vivo studies [125]. They allow for a more detailed view on

In vitro and in vivo interactions of selected nanoparticles with rodent serum proteins and their consequences in biokinetics

• Wolfgang G. Kreyling,
• Stefanie Fertsch-Gapp,
• Martin Schäffler,
• Blair D. Johnston,
• Nadine Haberl,
• Christian Pfeiffer,
• Jörg Diendorf,
• Carsten Schleh,
• Stephanie Hirn,
• Manuela Semmler-Behnke,
• Matthias Epple and
• Wolfgang J. Parak

Beilstein J. Nanotechnol. 2014, 5, 1699–1711, doi:10.3762/bjnano.5.180

• biokinetics of grafted AuNP conjugates. However, these results also suggest that more research is required to better understand the mechanisms of how these proteins and others mediate the passage across organ membranes. Precision-cut lung slices after intratracheal instillation of PVP-coated silver NP Several

• modulate subsequent bacterial infections of the lungs in a way that can only be determined by an experimental design like the one described below. For this purpose, thin slices from rat lungs, so-called precision-cut lung slices (PCLS), were employed. In PCLS the lung structure with all proteins and

• saline. Cylinders (8 mm diameter) of lung tissue were punched out and cut into 200 µm thick slices (precision-cut lung slices, PCLS). Four PCLS were incubated in full medium in each well of 24-well tissue culture plates. The plates were put into a 37 °C tissue culture incubator, and the melting agarose